LOCUS       MG209300                8357 bp    DNA     circular  SYN 
DEFINITION  Cel (tGFP-tagged) - Mouse carboxyl ester lipase (Cel)
ACCESSION   NM_009885
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2838..3542 
                     /label="GFP"  
     misc_feature    3589..4174 
                     /label="PolyA signal"  
     misc_feature    6266..6911 
                     /label="ColE1"  
     misc_feature    4628..5422 
                     /label="Neo"  
     misc_feature    4259..4561 
                     /label="SV40 ori"  
     misc_feature    6926..7786 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2817..2823 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2817 
                     /label="Cel"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCGCCTG GAGGTTCTAT TTCTTGGCCT CACCTGCTGC TTGGCAGCGG 
     1081 CTTGTGCTGC AAAGTTGGGG GCTGTGTACA CAGAAGGCGG TTTCGTGGAG GGCGTCAACA 
     1141 AGAAGCTCAG TCTCTTGGGT GGTGACTCTG TTGACATCTT CAAGGGTATC CCCTTTGCCA 
     1201 CTGCCAAGAC CTTGGAGAAT CCTCAGCGTC ACCCTGGCTG GCAAGGGACG CTGAAGGCTA 
     1261 CAAACTTCAA GAAACGATGC CTACAGGCCA CCATCACCCA GGACAACACC TATGGGCAAG 
     1321 AAGACTGCCT CTACCTCAAT ATCTGGGTGC CCCAGGGCAG GAAGCAGGTG TCCCACAACC 
     1381 TGCCTGTGAT GGTCTGGATC TATGGAGGTG CCTTCCTCAT GGGATCTGGC CAAGGGGCCA 
     1441 ATTTTCTCAA AAACTACCTG TATGACGGGG AGGAGATCGC CACTCGGGGC AACGTCATTG 
     1501 TGGTCACCTT CAACTACCGT GTTGGACCCT TGGGTTTCCT TAGCACCGGG GATGCTAACC 
     1561 TTCCAGGTAA CTTTGGTCTT CGAGATCAGC ACATGGCTAT CGCCTGGGTG AAGAGGAACA 
     1621 TTGCAGCCTT TGGGGGGGAC CCTGATAACA TCACCATCTT TGGGGAATCT GCTGGAGCTG 
     1681 CCAGTGTCTC CCTGCAGACC CTCTCCCCAT ACAACAAGGG TCTCATCCGG AGAGCCATCA 
     1741 GTCAGAGTGG TATGGCACTG AGCCCCTGGG CCATCCAGAA GAACCCACTT TTCTGGGCCA 
     1801 AAACGATTGC TAAGAAGGTA GGATGCCCCA CAGAGGATAC TGGCAAGATG GCTGCGTGTC 
     1861 TGAAGATCAC AGATCCCCGG GCCTTGACAC TGGCCTACAA GTTGCCCGTG AAAAAGCAGG 
     1921 AGTACCCTGT TGTGCACTAC CTGGCCTTCA TCCCTGTCAT CGATGGTGAC TTCATTCCCG 
     1981 ATGATCCCAT CAACCTATAC AACAACACTG CTGACATTGA CTACATAGCG GGCATTAACA 
     2041 ACATGGACGG CCACCTATTT GCTACCATTG ATGTGCCCGC TGTCGACAAG ACCAAGCAGA 
     2101 CTGTCACCGA GGAGGACTTC TACAGGCTAG TCAGTGGACA CACTGTCGCC AAGGGGCTGA 
     2161 AGGGGGCCCA AGCCACCTTT GACATCTACA CTGAGTCCTG GGCCCAGGAC CCGTCCCAGG 
     2221 AGAACATGAA GAAGACAATG GTGGCCTTTG AGACTGACGT ACTCTTCCTG ATCCCCACGG 
     2281 AGATGGCGCT GGCCCAGCAC AAAGCCCATG CCAAGAGCGC CAAGACCTAT TCTTACCTGT 
     2341 TTTCCCACCC TTCACGGATG CCTATCTACC CCAAATGGAT GGGGGCAGAC CATGCTGATG 
     2401 ACCTCCAGTA CGTCTTTGGA AAGCCTTTTG CCACCCCACT GGGCTACCGG CCCCAAGATA 
     2461 GGGCTGTCTC CAAGGCCATG ATCGCCTATT GGACCAACTT TGCCAGGAGT GGGGACCCCA 
     2521 ACATGGGCAA CTCACCTGTG CCCACACACT GGTACCCTTA TACCTTGGAG AATGGCAACT 
     2581 ACCTGGACAT CACTAAGACG ATAACCAGCG CTTCCATGAA GGAGCACCTG AGAGAAAAGT 
     2641 TCCTGAAGTT CTGGGCTGTG ACATTCGAGG TGCTGCCCAC AGTGACTGGT GACCAGGACA 
     2701 CCCTCACTCC CCCTGAGGAT GACTCAGAGG TTGCCCCTGA CCCACCTTCA GACGACTCCC 
     2761 AGGTTGTTCC TGTCCCACCT ACAGATGACT CTGTGGAGGC TCAGATGCCT GCCACCATTG 
     2821 GCTTCACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     2881 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     2941 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3001 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     3061 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     3121 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     3181 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     3241 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     3301 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     3361 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     3421 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     3481 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     3541 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3601 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3661 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3721 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3781 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3841 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3901 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3961 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4021 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4081 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4141 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4201 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     4261 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     4321 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4381 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     4441 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     4501 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     4561 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     4621 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     4681 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     4741 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     4801 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     4861 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     4921 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     4981 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     5041 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     5101 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     5161 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     5221 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     5281 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     5341 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     5401 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     5461 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     5521 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     5581 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     5641 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     5701 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     5761 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     5821 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     5881 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     5941 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6001 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     6061 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     6121 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     6181 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     6241 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     6301 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     6361 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     6421 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     6481 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     6541 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     6601 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     6661 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     6721 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     6781 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     6841 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     6901 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     6961 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7021 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     7081 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     7141 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     7201 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     7261 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     7321 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     7381 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     7441 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     7501 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     7561 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     7621 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     7681 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     7741 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     7801 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     7861 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     7921 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     7981 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     8041 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     8101 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     8161 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     8221 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     8281 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     8341 AATTTAACGC GAATTTT
//