LOCUS       MG208315                8117 bp    DNA     circular  SYN 
DEFINITION  Aldh2 (tGFP-tagged) - Mouse aldehyde dehydrogenase 2, mitochondrial (Aldh2)
ACCESSION   NM_009656
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2598..3302 
                     /label="GFP"  
     misc_feature    3349..3934 
                     /label="PolyA signal"  
     misc_feature    6026..6671 
                     /label="ColE1"  
     misc_feature    4388..5182 
                     /label="Neo"  
     misc_feature    4019..4321 
                     /label="SV40 ori"  
     misc_feature    6686..7546 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2577..2583 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2577 
                     /label="Aldh2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCGCGCC GCACTCACCA CTGTCCGCCG CGGACCGCGC CTGAGCCGCC 
     1081 TGTTGTCCGC CGCCGCCACC AGCGCGGTGC CAGCCCCCAA CCATCAGCCT GAGGTCTTCT 
     1141 GCAACCAGAT CTTCATTAAC AATGAGTGGC ACGACGCCGT CAGCAGGAAA ACATTTCCCA 
     1201 CCGTCAACCC TTCCACAGGG GAGGTCATCT GCCAGGTGGC CGAAGGGAAC AAGGAGGACG 
     1261 TAGACAAGGC AGTGAAGGCT GCTCGTGCAG CCTTCCAGCT GGGCTCGCCC TGGCGCCGCA 
     1321 TGGATGCATC TGACCGGGGC CGGCTGTTGT ACCGATTGGC GGATCTCATT GAACGGGACC 
     1381 GGACCTACCT AGCGGCCTTG GAGACCCTGG ACAACGGCAA GCCTTATGTC ATCTCGTACC 
     1441 TGGTGGATTT GGACATGGTC CTGAAATGTC TCCGCTATTA CGCTGGCTGG GCTGACAAGT 
     1501 ACCATGGGAA AACCATTCCC ATCGACGGCG ACTTCTTCAG CTATACCCGC CATGAGCCTG 
     1561 TGGGCGTGTG TGGACAGATC ATTCCGTGGA ACTTCCCGCT CCTGATGCAA GCATGGAAAC 
     1621 TGGGCCCAGC CCTGGCAACC GGGAACGTGG TGGTGATGAA GGTGGCCGAG CAGACACCGC 
     1681 TCACCGCGCT CTACGTGGCC AACTTGATCA AGGAGGCAGG CTTTCCCCCT GGCGTGGTCA 
     1741 ATATCGTTCC CGGATTCGGC CCTACCGCCG GGGCTGCCAT CGCATCCCAT GAGGGTGTGG 
     1801 ACAAAGTGGC GTTCACAGGC TCCACGGAGG TTGGTCACCT AATCCAGGTG GCCGCCGGGA 
     1861 GCAGCAACCT CAAGAGAGTA ACCCTGGAGC TGGGGGGAAA GAGTCCCAAC ATCATCATGT 
     1921 CCGACGCTGA CATGGACTGG GCTGTGGAGC AGGCCCACTT TGCCCTGTTC TTCAACCAGG 
     1981 GCCAGTGCTG CTGCGCAGGC TCCCGGACCT TCGTGCAGGA GAATGTGTAT GACGAATTCG 
     2041 TGGAACGCAG CGTGGCTCGG GCCAAGTCTC GGGTGGTGGG GAACCCCTTC GACAGCCGGA 
     2101 CGGAGCAGGG GCCTCAGGTG GATGAAACTC AGTTTAAGAA GATCCTCGGC TACATCAAAT 
     2161 CGGGACAACA AGAAGGGGCG AAGCTGCTGT GTGGTGGGGG CGCTGCCGCG GACCGTGGCT 
     2221 ACTTTATCCA GCCCACCGTG TTCGGGGACG TAAAAGACGG CATGACCATT GCCAAGGAGG 
     2281 AGATCTTTGG ACCAGTGATG CAAATCCTCA AATTCAAGAC CATCGAGGAG GTTGTGGGGC 
     2341 GGGCCAATGA TTCTAAGTAT GGGCTGGCAG CCGCCGTCTT CACAAAGGAC CTGGATAAAG 
     2401 CCAATTACCT GTCCCAAGCT CTGCAGGCTG GCACTGTGTG GATCAACTGC TACGATGTGT 
     2461 TTGGGGCCCA GTCTCCATTT GGGGGCTATA AGATGTCAGG GAGTGGCAGG GAGCTGGGCG 
     2521 AGTATGGCCT GCAGGCGTAC ACAGAAGTGA AGACGGTTAC TGTCAAAGTG CCACAGAAGA 
     2581 ACTCGACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     2641 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     2701 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     2761 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     2821 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     2881 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     2941 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     3001 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     3061 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     3121 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     3181 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     3241 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     3301 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3361 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3421 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3481 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3541 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3601 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3661 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3721 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3781 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3841 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3901 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     3961 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     4021 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     4081 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4141 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     4201 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     4261 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     4321 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     4381 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     4441 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     4501 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     4561 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     4621 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     4681 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     4741 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     4801 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     4861 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     4921 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     4981 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     5041 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     5101 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     5161 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     5221 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     5281 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     5341 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     5401 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     5461 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     5521 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     5581 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     5641 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     5701 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     5761 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     5821 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     5881 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     5941 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     6001 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     6061 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     6121 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     6181 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     6241 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     6301 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     6361 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     6421 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     6481 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     6541 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     6601 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     6661 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     6721 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     6781 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     6841 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     6901 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     6961 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     7021 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     7081 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     7141 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     7201 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     7261 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     7321 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     7381 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     7441 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     7501 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     7561 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     7621 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     7681 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     7741 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     7801 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     7861 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     7921 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     7981 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     8041 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     8101 AATTTAACGC GAATTTT
//