LOCUS       MG208192                8087 bp    DNA     circular  SYN 
DEFINITION  Nek3 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 3 (Nek3)
ACCESSION   NM_011848
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2568..3272 
                     /label="GFP"  
     misc_feature    3319..3904 
                     /label="PolyA signal"  
     misc_feature    5996..6641 
                     /label="ColE1"  
     misc_feature    4358..5152 
                     /label="Neo"  
     misc_feature    3989..4291 
                     /label="SV40 ori"  
     misc_feature    6656..7516 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2547..2553 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2547 
                     /label="Nek3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAACTAC ACAGTCCTGA GAGTGATTGG CCAGGGCTCC TTCGGCAGGG 
     1081 CTCTTTTGGT TCTGCAGGAA AGCAGCAATC AGACATTTGC CATGAAGGAA ATCAGACTGC 
     1141 TCAAGTCTGA TACACAGACT TCTAGGAAAG AGGCTGTTCT GCTCGCCAAA ATGAAGCACC 
     1201 CCAATATTGT TGCCTTTAAG GAGTCATTTG AAGCTGAAGG GTACCTGTAC ATTGTGATGG 
     1261 AGTATTGTGA CGGAGGGGAT CTGATGCAGA GGATTAAACA GCAAAAAGGG AAGTTATTCC 
     1321 CCGAAGACAC GATACTTAAT TGGTTTATAC AAATTTGTCT TGGAGTAAAT CACATTCATA 
     1381 AGAGACGTGT TTTACACAGA GATATCAAGT CGAAAAATGT CTTCCTCACC CACAATGGAA 
     1441 AAGTGAAATT GGGAGATTTT GGCTCTGCCC GTCTTCTCTC CAGTCCCATG GCATTTGCAT 
     1501 GCACGTACGT GGGGACACCT TATTATGTGC CTCCAGAAAT CTGGGAGAAC CTGCCTTACA 
     1561 ACAATAAAAG TGACATCTGG TCCTTGGGAT GCATTCTGTA TGAACTCTGC GCTCTGAAGC 
     1621 ATCCATTCCA GGCAAATAGT TGGAAAAACC TGATCCTGAA AATATGCCAG GGGCCCATCC 
     1681 ACCCGCTGCC GGCTCTATAC TCCTGCAAGC TTCAGGACCT GGTCAAGCAG ATGCTGAAAA 
     1741 GGAACCCTTC GCACCGCCCC TCCGTCACAA CCCTGCTCTG CAGGGGTAGC TTGGCTCCAT 
     1801 TGGTCCTGAA GTGCTTGCCC CCTCAGATCA TCAGAGAATA TGGAGAACAG ATATTAGATG 
     1861 AAATCAAAAT ATCCACGCCT AAGAATATGA AAAAACAGGA CTCCAACAGA GTCGGGAGAG 
     1921 CTCTGGGAGA AGCAAACTCT GCCTCTATGC AAGAGGAAGA ACGTGGTAGA AAGTGTAGCC 
     1981 ATACTGAATT GGAAAGCACT GGTACAACCC CAGCTGGGAA TGCATTGGGA AGAGCAGCCA 
     2041 GAGGAAATCC AGGTAACCCA CAGGAGCACG GGAGGCATAC CAGTCCGGCA AGTCCTCACA 
     2101 GGCCGCGGTG GGAGAGACAC GGTCCCAGTT CCAATGTCGA AGCTTTGGAG AAAGCATCCA 
     2161 TCCTTACCTC CAGTTTCACG GCAGAGGATG ACAGAGGTGG GTCTGTGATA AAGTATGAAG 
     2221 AAAATGCCCG AAGGCAGTGG GTCAGAGAGC CCCCTGAGGC CTTGCTGTCA ATGCTGAAGG 
     2281 ACGCTGATCT CAGTCAGGCA TTTCAAACAT ACACAATATA TAGACCAGGT GCAGAAGGAT 
     2341 TCCTGAAAGG TCCCCTTTCT GAAGACACGG CATCGGACAG CGTCGACGGT GATCTCGATT 
     2401 CTGTCATGTT AGACCCAGAG AGATTTGAAC CTCGGCTGGA TGAAGAAGAC ACGGACTTTG 
     2461 AGGAAGATAA CGAAAACCCT GATTGGCTGT CAGAACTAAA GAAGCACGTA GGATACGGGG 
     2521 ATGGACCTGG TGGACAACTC CTCGGAGAAT GTGCCACGCG TACGCGGCCG CTCGAGATGG 
     2581 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     2641 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     2701 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     2761 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     2821 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     2881 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     2941 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     3001 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     3061 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     3121 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     3181 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     3241 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     3301 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3361 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3421 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3481 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3541 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3601 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3661 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3721 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3781 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3841 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     3901 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     3961 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     4021 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     4081 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     4141 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     4201 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     4261 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     4321 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     4381 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     4441 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     4501 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     4561 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     4621 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     4681 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     4741 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     4801 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     4861 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     4921 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     4981 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     5041 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     5101 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     5161 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     5221 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     5281 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     5341 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     5401 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     5461 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     5521 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     5581 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     5641 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     5701 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     5761 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     5821 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     5881 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     5941 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     6001 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     6061 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     6121 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     6181 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     6241 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     6301 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     6361 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     6421 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     6481 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     6541 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     6601 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     6661 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     6721 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     6781 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     6841 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     6901 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     6961 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     7021 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     7081 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     7141 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     7201 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     7261 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     7321 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     7381 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     7441 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     7501 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     7561 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     7621 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     7681 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     7741 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     7801 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     7861 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     7921 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     7981 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     8041 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//