LOCUS       MG207436                7955 bp    DNA     circular  SYN 
DEFINITION  Pnlip (tGFP-tagged) - Mouse pancreatic lipase (Pnlip)
ACCESSION   NM_026925
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2436..3140 
                     /label="GFP"  
     misc_feature    3187..3772 
                     /label="PolyA signal"  
     misc_feature    5864..6509 
                     /label="ColE1"  
     misc_feature    4226..5020 
                     /label="Neo"  
     misc_feature    3857..4159 
                     /label="SV40 ori"  
     misc_feature    6524..7384 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2415..2421 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2415 
                     /label="Pnlip"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAATGCTG TGGACATTTG CAGTGCTGCT GGGAGCAGTA GCTGGAAGGG 
     1081 AAGTTTGCTT CGACAAACTC GGCTGCTTCA GTGATGATGC CCCCTGGTCA GGAACTCTAG 
     1141 ACAGACCCCT GAAAGCACTG CCCTGGAGCC CAGCACAGAT CAACACCCGC TTTCTCCTCT 
     1201 ACACCAACGA GAATCCAGAC AACTACCAGC TAATTACTTC AGATGCATCA AACATCAGAA 
     1261 ACTCCAATTT CCGAACAAAC AGAAAAACCC GTATCATTAT CCACGGCTTC ATCGACAAGG 
     1321 GAGAAGAAAA CTGGCTATCT GACATGTGCA AGAACATGTT CCGAGTGGAG AGTGTGAACT 
     1381 GCATCTGTGT GGACTGGAAA GGCGGTTCCC GAACGACATA TACCCAGGCT ACCCAGAACG 
     1441 TCCGGGTCGT GGGGGCAGAA GTGGCATTGT TGGTTAACGT TCTGCAGTCT GATCTGGGAT 
     1501 ACTCACTTAA CAATGTCCAC CTGATTGGCC ACAGCCTGGG TTCCCACATT GCTGGGGAGG 
     1561 CTGGAAAGAG GACCTTTGGA GCCATTGGAA GGATCACAGG GTTGGACCCC GCTGAGCCTT 
     1621 ACTTCCAGGG CACCCCTGAA GAAGTCCGAC TGGACCCCAC CGATGCTCAG TTTGTGGACG 
     1681 CAATTCACAC AGACGCCGGG CCCATTATCC CCAACTTAGG ATTTGGAATG AGCCAGACTG 
     1741 TGGGTCACTT AGATTTCTTT CCAAACGGAG GAATCGAAAT GCCAGGATGC CAGAAGAATA 
     1801 TTCTTTCCCA AATTGTTGAC ATCGACGGGA TCTGGGAAGG AACTCGAAAC TTTGCTGCCT 
     1861 GTAACCACTT AAGAAGCTAC AAGTTTTACA CCGACAGCAT CGTCAACCCA ACTGGCTTTG 
     1921 CTGGGTTCTC CTGTAGCTCT TACAGTCTTT TCACGGCAAA TAAGTGCTTC CCATGTGGGA 
     1981 GTGGAGGATG CCCCCAGATG GGTCATTACG CTGACAGATA TCCTGGGAAA ACAAGCAGAC 
     2041 TCTACCAGAC ATTTTACCTC AACACCGGTG ATAAGAGCAA CTTCGCACGT TGGCGGTACC 
     2101 AGGTGACTGT CACCCTGTCT GGACAGAAGG TCACTGGGCA CATTCTAGTT TCTTTGTTTG 
     2161 GAAATGGAGG AAACTCTAAA CAGTATGAAG TTTTCAAGGG CTCTCTGCAG CCAGGTACTT 
     2221 CTCACGTCAA TGAATTCGAC TCTGATGTGG ATGTTGGAGA TTTGCAGAAG GTTAAATTTA 
     2281 TTTGGTACAA CAATGTGATC AACCCAACTC TACCCAAAGT GGGAGCATCA AGGATCACAG 
     2341 TGGAAAGAAA TGATGGCAGA GTGTTCAACT TCTGTAGTCA AGAGACAGTG AGGGAAGACG 
     2401 TCCTGCTCAC ACTGTCTCCA TGTACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     2461 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     2521 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     2581 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     2641 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     2701 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     2761 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     2821 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     2881 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     2941 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     3001 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     3061 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     3121 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3181 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3241 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3301 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3361 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3421 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3481 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3541 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3601 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3661 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3721 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3781 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     3841 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     3901 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     3961 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     4021 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     4081 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     4141 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     4201 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     4261 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     4321 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     4381 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     4441 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     4501 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     4561 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     4621 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     4681 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     4741 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     4801 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     4861 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     4921 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     4981 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     5041 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     5101 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     5161 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     5221 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     5281 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     5341 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     5401 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     5461 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     5521 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     5581 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     5641 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     5701 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     5761 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     5821 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     5881 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     5941 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     6001 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     6061 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     6121 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     6181 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     6241 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     6301 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     6361 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     6421 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     6481 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     6541 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     6601 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     6661 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     6721 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     6781 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     6841 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     6901 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     6961 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     7021 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     7081 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     7141 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     7201 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     7261 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     7321 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     7381 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     7441 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     7501 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     7561 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     7621 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     7681 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     7741 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     7801 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     7861 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     7921 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//