LOCUS MC221298 7140 bp DNA circular SYN DEFINITION Folh1 (untagged) - Mouse folate hydrolase (Folh1), transcript variant 1, (10ug) ACCESSION NM_016770 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3297..3388 /label="Myc-DDK" misc_feature 3418..4003 /label="PolyA signal" misc_feature 3475..3494 /label="Restriction Enzyme" misc_feature 4279..4951 /label="ColE1" misc_feature 5381..6175 /label="Kan/Neo" misc_feature 6210..6512 /label="SV40 ori" misc_feature 6598..7075 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3279..3285 /label="Restriction Enzyme" misc_feature 1020..3279 /label="Folh1" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GTGGAACGCA CTGCAGGACA GAGACTCCGC GGAGGTCCTG GGACACCGCC 1081 AGCGCTGGCT CCGTGTTGGG ACACTGGTGC TGGCTTTAAC CGGAACCTTC CTCATTGGCT 1141 TCCTCTTTGG GTGGTTTATA AAACCTTCCA ATGAAGCTAC TGGTAATGTT TCCCATTCTG 1201 GCATGAAGAA GGAGTTTTTG CATGAATTGA AGGCTGAGAA CATCAAAAAA TTTTTATACA 1261 ATTTCACACG GACACCACAC TTGGCAGGAA CACAAAATAA TTTTGAGCTT GCAAAGCAAA 1321 TTCATGACCA GTGGAAAGAA TTTGGCCTGG ATTTGGTTGA GTTATCCCAT TACGATGTCT 1381 TGCTGTCCTA TCCAAATAAA ACTCATCCTA ACTATATCTC AATAATTAAT GAAGATGGAA 1441 ATGAGATTTT CAAAACATCA TTATCTGAAC AGCCACCCCC AGGATATGAG AATATATCAG 1501 ATGTAGTGCC ACCATACAGT GCCTTCTCTC CACAAGGGAC ACCAGAGGGT GATCTAGTGT 1561 ATGTCAACTA TGCACGAACT GAAGACTTCT TTAAACTGGA ACGGGAAATG AAGATCAGTT 1621 GTTCTGGGAA GATTGTGATT GCCAGATATG GGAAAGTGTT CAGAGGAAAT ATGGTTAAAA 1681 ATGCTCAACT GGCAGGGGCA AAAGGAATGA TTCTGTACTC AGACCCTGCT GACTACTTTG 1741 TTCCTGCGGT GAAGTCCTAT CCAGATGGCT GGAACCTCCC TGGAGGTGGT GTCCAACGTG 1801 GAAATGTCTT AAATCTTAAT GGTGCAGGTG ACCCGCTCAC ACCAGGTTAC CCAGCAAATG 1861 AACATGCTTA TAGGCATGAG TTGACAAACG CTGTTGGCCT TCCAAGTATT CCTGTCCATC 1921 CTATTGGATA TGATGATGCA CAGAAACTCT TAGAACACAT GGGTGGTCCA GCACCCCCTG 1981 ACAGTAGCTG GAAGGGAGGA TTAAAAGTGC CTTACAACGT GGGACCTGGC TTTGCTGGAA 2041 ACTTTTCAAC ACAAAAGGTC AAGATGCATA TTCACTCTTA CACTAAAGTG ACAAGAATCT 2101 ATAATGTCAT TGGCACCCTC AAAGGAGCTC TGGAACCAGA CAGATATGTT ATTCTTGGAG 2161 GTCACCGAGA TGCTTGGGTA TTTGGTGGCA TTGACCCTCA GAGTGGAGCA GCTGTTGTTC 2221 ATGAAATTGT GCGGAGCTTT GGAACCCTGA AGAAGAAAGG ACGGAGGCCT AGAAGGACAA 2281 TTTTGTTTGC AAGCTGGGAT GCAGAAGAAT TTGGCCTTCT TGGTTCTACT GAGTGGGCAG 2341 AGGAACATTC AAGACTCCTA CAAGAGCGAG GTGTGGCTTA TATTAATGCT GATTCTTCCA 2401 TAGAAGGAAA TTACACTCTA AGAGTTGATT GCACACCACT GATGTACAGC TTAGTGTACA 2461 ACCTAACAAA AGAGCTGCAA AGCCCAGATG AAGGTTTTGA AGGAAAATCT CTTTATGACA 2521 GCTGGAAAGA AAAGAGTCCT TCACCTGAGT TCATTGGAAT GCCCAGAATT AGCAAGCTGG 2581 GGTCTGGCAA TGATTTTGAA GTGTTCTTCC AAAGACTTGG AATTGCTTCA GGCAGAGCCC 2641 GATATACTAA AAATTGGAAA ACTAACAAAG TCAGCAGCTA TCCTCTCTAT CACAGTGTCT 2701 ATGAAACATA TGAGCTGGTA GTAAAATTTT ATGACCCAAC ATTTAAATAC CACCTCACTG 2761 TGGCCCAGGT TCGAGGAGCA ATGGTATTTG AACTTGCCAA TTCTATAGTG CTTCCCTTTG 2821 ACTGCCAAAG TTATGCTGTA GCTCTGAAGA AGTATGCTGA CACTATCTAC AATATTTCAA 2881 TGAAACATCC ACAAGAAATG AAGGCTTACA TGATATCATT TGATTCACTG TTTTCTGCAG 2941 TCAATAATTT TACAGATGTT GCATCTAAGT TCAATCAGAG ACTGCAAGAG TTAGACAAAA 3001 GCAACCCCAT ATTACTGAGA ATTATGAATG ACCAGCTGAT GTATCTGGAA CGTGCATTCA 3061 TTGATCCTTT AGGCTTACCA GGAAGGCCTT TCTACAGGCA TATCATCTAT GCTCCAAGCA 3121 GCCACAACAA GTATGCAGGA GAATCATTCC CTGGGATTTA TGATGCCCTT TTTGATATAA 3181 GTAGCAAAGT CAATGCTTCT AAGGCCTGGA ACGAAGTGAA GAGACAGATT TCTATTGCAA 3241 CCTTTACAGT GCAAGCTGCA GCAGAGACTC TGAGGGAAGT AGCTTAAACG CGTACGCGGC 3301 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 3361 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 3421 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 3481 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 3541 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 3601 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 3661 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 3721 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 3781 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 3841 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 3901 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 3961 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 4021 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 4081 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 4141 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 4201 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 4261 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 4321 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 4381 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 4441 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 4501 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 4561 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 4621 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 4681 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 4741 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 4801 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 4861 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 4921 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 4981 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 5041 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 5101 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 5161 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 5221 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 5281 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 5341 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 5401 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 5461 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 5521 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 5581 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 5641 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 5701 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 5761 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 5821 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 5881 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 5941 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 6001 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 6061 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 6121 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 6181 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 6241 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 6301 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 6361 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 6421 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 6481 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 6541 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 6601 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 6661 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 6721 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 6781 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 6841 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 6901 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 6961 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 7021 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 7081 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT //